IN-VITRO FERTILIZATION (IVF)
* 1 straw of semen can fertilize several donors
* About 10 – 15 eggs per collection. About 73% of these will be fertilized
45 - 55% will become a viable and freezable
* Pregnancy rate with IVF embryos = Average 45 - 60% (depending on fresh or frozen implant)
* Collection of donors every 2 weeks
Donors can be collected pregnant up to 120 days of pregnancy
* Can collect heifers either before or after breeding
* If conventional flushing not working, IVF may be a viable option to produce good embryos
A growing number of cattle breeders are using In-Vitro Fertilization (IVF) to maximize embryo production from donors that need to be pregnant, problem cows, young heifers and better use of expensive or rare semen.
This is the process of harvesting oocytes from donor cow’s ovaries, and creating embryos by fertilizing the oocytes with semen in a petri dish.
The embryo is then implanted into a recipient cow (surrogate cow), or they can be frozen indefinitely.
IVF is a proven technique with sound conception rates when performed correctly, conventional ET is less expensive than IVF. However, that is changing, as IVF costs are decreasing with different lab methods. With conventional ET, a donor female needs to be kept open to be bred and flushed, and cows can be collected at a minimum of every 30 days.
Collections every two weeks are possible with IVF, as the eggs are collected directly off the ovaries, not from the uterus. This also allows a person to collect from pregnant cows. After confirming by ultrasound that they’re 30 to 35 days in calf, the female receives the Folltropin super-stimulation drug to produce multiple follicles. The IVF veterinarian then uses an ultrasound-guided needle to aspirate the follicles off the ovaries. In the lab, follicles are matured and fertilized before being cultured for seven days.
HOW THE PROCEDURE WORKS
The first stage is the key addition to conventional embryo transfer. Eggs are removed from the donor cows ovary before the ovary naturally releases the oocyte down the fallopian tube.
Using a process called trans-vaginal aspiration, IVF works by first removing the dominant follicle in the ovary, allowing the rest to grow.
In a normal estrous cycle, the dominant follicle inhibits the rest – otherwise a cow could end up developing dozens of follicles and end up with a pregnancy with multiple calves. With IVF, all follicles are left for six days to develop uninhibited within the ovary by performing a process called dominant follicle removal (DFR).
1. Dominant Follicle Removal (DFR)
The donor cow is given a local anesthetic and cleaned with a mild disinfectant and saline solution. This can happen on-farm or at the Bova-Tech facility.
An ultrasound-guided needle enters the ovary to remove the dominant follicle to stimulate super-ovulation.
A three-day course of follicle stimulating hormones (FSH) is administered to stimulate ovaries to produce more oocytes.
3. Ovum pick-up (OPU)
The donor cow is brought into the collection facility, where the temperature is at 27-32C. Oocytes are harvested via trans-vaginal aspirations, averaging 10 - 15 eggs per collection depending on the donor cow, the eggs are then matured for 20-24 hours.
4. Insulated chambers
Eggs are placed into insulated chambers between 37C and 38C to mimic a cow’s body temperature.
5. Fertilization occurs with semen
One straw of semen can be utilized to fertilize several eggs from different cows if necessary.
Resulting embryos are matured for a week in the laboratory, passing through eight different maturation liquids (medias) mimicking the changing pH and gas levels inside the uterus.
7. Embryos can be transferred directly or frozen indefinitely
Problem breeders can be salvaged because they can be used as a donor if, for example, they have:
Fallopian tubes blocked
Extend breeding life:
Collection possible from 10 months to 20 years old and above
Ovaries can be recovered from abattoir